To prepare samples for RNA sequencing, strain JA was cultured on a compost purchased from a company producing button mushroom (Jinming Food Co., Ltd, Fujian, China), and the main components of the compost are rice straw, bagasse, cow manure, and gypsum. blazei strain JA, the main cultivar in several provinces in China (eg., Fujian, Yunnan, Jiangxi, Guangxi, Henan, and Sichuan province), was provided by Fujian Academy of Agricultural Sciences. Our genomic and transcriptomic data will provide important information platform for the further investigation of this species. The transcriptome analysis will enable us to identify genes regulating mycelia growth and fruiting body formation. Additionally, we also performed the transcriptome analysis of mycelia and primordia to study the expression difference between the two stages. blazei to understand the genomic structure and its gene content. In this study, we presented the genome of A. Understanding the molecular mechanism regulating fruiting body formation will contribute to the improvement of commercial mushroom production, with consequent economic benefits. The shift from vegetative mycelia to primordia has been seen as one of the most complex and critical developmental processes in mushrooms. Additionally, transcriptome or comparative expression profiles from different developmental stages have facilitated the discovery of genes involved in fruiting body formation. The availability of genomes has been a benefit to researchers for identification of CAZymes, mating-type loci, β-glucan synthase genes, and secondary metabolite genes. Recently, several studies reported genome or transcriptome landscape of mushrooms, such as Agaricus bisporus, Volvariella volvacea, Flammulina velutipes, and Auricularia heimuer. However, very few studies focus on those compounds’ biosynthesis pathways and the fruiting body formation in A. Moreover, several volatile flavor ingredients have been identified in its fruiting body and mycelia.
#Primordia bridge code series#
A series of bioactive compounds, for instance polysaccharides, lectin, and ergosterol, have been discovered in A.
blazei has received great scientific and practical interest. Also, it has been widely consumed today in several Oriental countries and studied for its high nutritional properties and pharmacological effects, such as antitumor activity, antiviral effect, antidiabetic potential, and antiapoptotic role. It serves as one of the utmost valuable edible and culinary-medicinal Royal Sun mushroom species. Agaricus blazei Murrill, an edible mushroom originating from Brazil, is commonly known as “Cogumelo do Sol” in Brazil, “Himematsutake” in Japan, and “Ji Song Rong” in China. Mushrooms (mainly the fruiting body) have been used for consumption as a product by humans since ancient times due to their delicacy and nutritional values, as well as their medicinal properties. blazei provide valuable sequence resources and contribute to our understanding of genes related to the biosynthesis pathway of polysaccharide and benzaldehyde, as well as the fruiting body formation. The genome and transcriptome analysis of A. Functional enrichment showed that differentially expressed genes associated with response to stress, ribosome biogenesis, arginine biosynthesis, and steroid biosynthesis pathway were more active in fruiting body. Expression profiling analysis revealed that 2,164 genes were upregulated in mycelia and 1,557 in primordia. Additionally, we also studied transcriptional regulation of the mycelia and the primordia for exploration of genes involved in fruiting body formation. De novo assembly generated 36 contigs with 38,686,133 bp in size, containing 10,119 putative predicted genes. High-throughput Illumina PE150 and PacBio RSII platform were employed to generate a genomic sequence.
Agaricus blazei, a type of edible straw-rotting mushroom with somewhat sweet taste and fragrance of almonds, has attracted considerable scientific and practical attention.
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